lplJ [2019-05-09 09:59:53]

lipoate-protein ligase

locus: BSU10250

pI: 5.84

mw: 37.86 kDa

protein length: 331 aa

gene length: 996 bp

function: scavenging of lipoic acid

product: Lipoate:protein ligase

essential: no

synonyms: yhfJ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]

Gene

Coordinates: 1,099,159 → 1,100,154

The protein

Catalyzed reaction/ biological activity

• attaches exogenous lipoic acid to the apoproteins by a two-step ATP

Structure

• [PDB|5IBY] (from Enterococcus faecalis, 59% identity)

Expression and Regulation

Operons

• genes: [gene|8FA225FA26DB0B89812A835EB928F2C8892EBC89|yhfI]-[gene|F68611DF326880FCCB4EE3C2B364ECCC3DEAD78D|lplJ]-[gene|2DA0641FA6EC81072DA585D65FE17B7E5A010AC9|yhfK]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B276 (yhfJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1275 NBRP B. subtilis, Japan]
• BKE10250 (Δ[gene|F68611DF326880FCCB4EE3C2B364ECCC3DEAD78D|lplJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATAAATAACATGGTGCTCC, downstream forward: _UP4_TAAGGCAAAACACATCGTTT
• BKK10250 (Δ[gene|F68611DF326880FCCB4EE3C2B364ECCC3DEAD78D|lplJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATAAATAACATGGTGCTCC, downstream forward: _UP4_TAAGGCAAAACACATCGTTT

References

Reviews

• 27074917

Original publications

• 21338420,21338421